C. DIFFICILE TOXINS A & B PROFILE | SENGKANG GENERAL HOSPITAL

Lab Section Category	Microbiology
Specimen Collection	Unformed Stool in stool container* *No sharing with other tests; Minimum of one tablespoon
Storage/Transport	• Specimen should reach the Microbiology Laboratory as soon as possible. • If unable to dispatch within the same day, specimens should be kept at 2 to 8°C.
Methodology	For Diagnosis of C. difficile infection: Rapid Membrane Enzyme Immunoassay (C. Diff QuikChek Complete) for detection of: • Glutamate Dehydrogenase (GDH) • Toxins A + B A reflex PCR test (Cepheid Xpert C. difficile/epi) will be performed when sample is positive for GDH but negative for toxins A + B (separately charged).
Test Result	Reporting: • Detected • Not Detected • Inconclusive
Turnaround Time	24 hours
Day(s) Test Set Up	Daily (incl. Sundays and Public Holidays)
Additional Information	-
Sample Rejection	The following specimens will be rejected: • Leaked specimens • Stool mixed with urine • Stool in eSwab • Insufficient specimen • More than 3 days after collection

Last Updated - 09 Mar 2026